Calpastatin 抗体 (Middle Region)
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- 抗原 See all Calpastatin (CAST) 抗体
- Calpastatin (CAST)
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抗原表位
- AA 275-310, Middle Region
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Calpastatin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Calpastatin(CAST) detection. Tested with WB, IHC-P in Human.
- 序列
- QEKKRKVEKD TMSDQALEAL SASLGTRQAE PELDLR
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Calpastatin(CAST) detection. Tested with WB, IHC-P in Human.
Gene Name: calpastatin
Protein Name: Calpastatin - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin (275-310aa QEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLR), different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product CAST Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- Calpastatin (CAST)
- 别名
- CAST (CAST 产品)
- 别名
- BS-17 antibody, calp3 antibody, CAST antibody, sb:cb966 antibody, zgc:194249 antibody, cast antibody, DKFZp469E0716 antibody, calpastatin antibody, calpastatin S homeolog antibody, Calpastatin antibody, CAST antibody, Cast antibody, cast.S antibody, cast antibody, VDBG_09182 antibody, ical antibody
- 背景
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Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Synonyms: BS 17 | Calpain inhibitor | Calpastatin | Cast | PLACK | Sperm BS 17 component | Sperm BS-17 component | P20810 - 基因ID
- 831
- UniProt
- P20810
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