Toc75 抗体
This anti-Toc75 antibody is a 兔 多克隆 antibody detecting Toc75 in FACS, IL 和 WB. Suitable for Pisum sativum, Synechococcus sp., Thermosynechococcus 和 Synechocystis.
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Quick Overview for Toc75 抗体 (ABIN488514)
抗原
Toc75
(Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
适用
Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis
宿主
兔
克隆类型
多克隆
标记
This Toc75 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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预测反应
- Ricinus communis, Vitis vinifera, Populus trichocarpa
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
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纯化方法
- serum
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免疫原
- psTOC75, Predicted POTRA Domain #1, Amino acids, 158-241, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
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anti-Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75) (Chloroplastic), (Domain 3) antibody
Toc75 适用: Pisum sativum, Synechococcus sp., Thermosynechococcus, Arabidopsis thaliana, Nicotiana tabacum, Spinach FACS, IL, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
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说明
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antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
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背景
- OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
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分子量
- expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
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基因ID
- 158241
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UniProt
- Q43715
抗原
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