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Toc75 抗体

Toc75 适用: Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis FACS, IL, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN488514
发货至: 中国
  • 抗原 See all Toc75 products
    Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
    适用
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis
    宿主
    • 1
    克隆类型
    • 1
    多克隆
    标记
    • 1
    This Toc75 antibody is un-conjugated
    应用范围
    Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)
    交叉反应 (详细)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    预测反应
    Ricinus communis, Vitis vinifera, Populus trichocarpa
    产品特性
    Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
    纯化方法
    serum
    免疫原
    psTOC75, Predicted POTRA Domain #1, Amino acids, 158-241, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
  • 应用备注
    1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
    说明

    antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    For reconstitution add 200 μL of sterile water
    注意事项
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • 抗原
    Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
    Abstract
    Toc75 产品
    背景
    OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
    分子量
    expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
    基因ID
    158241
    UniProt
    Q43715
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