Toc75 抗体
The 兔 多克隆 anti-Toc75 antibody (ABIN488514) specifically detects Toc75 in FACS, IL 和 WB.
The antibody is reactive with Pea, Synechococcus sp., Synechocystis 和 Thermosynechococcus samples.
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产品编号 ABIN488514
发货至:
中国
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Quick Overview for Toc75 抗体 (ABIN488514)
抗原
Toc75
(Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
适用
Pea, Synechococcus sp., Synechocystis, Thermosynechococcus
宿主
兔
克隆类型
多克隆
标记
This Toc75 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)
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原理
- Anti-Toc75 | Protein TOC75-3, chloroplastic, POTRA domain 1
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交叉反应 （详细）
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Pisum sativum, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus
Not reactive in: Physcomitrella patens, Zea mays -
预测反应
- Oryza sativa,Ricinus communis,Populus trichocarpa,Vitis vinifera
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纯化方法
- Serum
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免疫原
- psTOC75, Predicted POTRA Domain #1, Amino acids
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anti-Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75) (Chloroplastic), (Domain 3) antibody
Toc75 适用: Pea, Synechococcus sp., Synechocystis, Thermosynechococcus, Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomytrium Patens, Spinach, Thellungiella salsuginea FACS, IL, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 500 (Flow cyt), 1 : 100 (IL), 1 : 2000-1 : 100 000 (WB)
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说明
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antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
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背景
- OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
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分子量
- 88 | 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
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基因ID
- 158241
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UniProt
- Q43715
抗原
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