Western blot: 1/500-1/3000. Positive Controls: 293T, A431, HeLa, HepG2. Immunofluorescence: 1/100-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
浓度
0.55 mg/mL
缓冲液
0.1 M Tris, 0.1 M Glycine, 10 % Glycerol ( pH 7), 0.01 % Thimerosal
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at -20 °C.
抗原
BAIAP2L1
(BAI1-Associated Protein 2-Like-1 (BAIAP2L1))
This gene encodes a member of the IMD (IRSp53/MIM homology domain) family. Members of this family can be subdivided in two groups, the IRSp53-like and MIM-like, based on the presence or absence of the SH3 (Src homology 3) domain. The protein encoded by this gene contains a conserved IMD, also known as F-actin bundling domain, at the N-terminus, and a canonical SH3 domain near the C-terminus, so it belongs to the IRSp53-like group. This protein is the substrate for insulin receptor tyrosine kinase and binds to the small GTPase Rac. It is involved in signal transduction pathways that link deformation of the plasma membrane and remodeling of the actin cytoskeleton. It also promotes actin assembly and membrane protrusions when overexpressed in mammalian cells, and is essential to the formation of a potent actin assembly complex during EHEC (Enterohemorrhagic Escherichia coli) pedestal formation. [provided by RefSeq].Synonyms: BAI1-associated protein 2-like 1, Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1, IRTKS, Insulin receptor tyrosine kinase substrate