This antibody is suitable for use in Immunofluorescence/Immunocytochemistry andWestern blotting. Recommended Dilutions: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200.
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
0.1 M Tris, 0.1 M Glycine, 10 % Glycerol and 0.01 % Thimerosal as preservative.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at -20 °C.
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families, beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3.Synonyms: Beta-A4 crystallin, CRYBA4