Peptide ELISA: 1/16000. Western blot: Approx 120 kDa band observed in Mouse and Rat Skeletal Muscle lysates(calculated MW of 130 kDa according to Mouse NP_080477.1 and Rat NP_001102005.1). Recommended concentration: 1 - 3 μg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. Thisband was successfully blocked by incubation with the immunizing peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
DIXDC1 belongs to the DIXDC1 family and contains 1 CH (calponin homology) domain and 1 DIX domain. The coiled coil domain mediates interaction with MAP3K4 and inhibition of AXIN1 mediated JNK activation through MAP3K4. The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1 mediated JNK activation through MAP3K1. Probably also mediates interaction with DVL2.Synonyms: CCD1, Coiled-coil protein DIX1, DIX domain-containing protein 1, DIX1, DIXDC1, KIAA1735