Kanamycin 抗体
This anti-Kanamycin antibody is a 绵羊 多克隆 antibody detecting Kanamycin in EIA. Suitable for Streptomyces kanamyceticus.
Quick Overview for Kanamycin 抗体 (ABIN487712)
抗原
Kanamycin
适用
Streptomyces kanamyceticus
宿主
绵羊
克隆类型
多克隆
标记
This Kanamycin antibody is un-conjugated
应用范围
Enzyme Immunoassay (EIA)
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纯化方法
- Ig Fraction
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免疫原
- Kanamycin-BTG
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亚型
- IgG
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anti-Kanamycin antibody
适用: 化学剂 ELISA 宿主: 小鼠 Monoclonal 3F7C11 unconjugated
anti-Kanamycin antibody适用: 化学剂 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 0.312 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10.77 mg/mL (by U.V. abs at 280nm)
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缓冲液
- 20 mM Phosphate, 150 mM Sodium Chloride, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Upon receipt, store undiluted (in aliquots) at-20 °C.
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- Kanamycin
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物质类
- Chemical
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背景
- Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 μg/mL) containing agar plates or are grown in media containing kanamycin (50-100 μg/mL). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/mL). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria, it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs.Synonyms: Kanamycin A, Kantrex
抗原
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