Kanamycin 抗体
适用: Streptomyces kanamyceticus
EIA
宿主: 绵羊
Polyclonal
unconjugated
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- 抗原 See all Kanamycin products
- Kanamycin
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适用
- Streptomyces kanamyceticus
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- This Kanamycin antibody is un-conjugated
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应用范围
- Enzyme Immunoassay (EIA)
- 纯化方法
- Ig Fraction
- 免疫原
- Kanamycin-BTG
- 亚型
- IgG
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anti-Kanamycin antibody
适用: 化学剂 ELISA 宿主: 小鼠 Monoclonal 3F7C11 unconjugated
anti-Kanamycin antibody适用: 化学剂 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 0.312 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 10.77 mg/mL (by U.V. abs at 280nm)
- 缓冲液
- 20 mM Phosphate, 150 mM Sodium Chloride, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Upon receipt, store undiluted (in aliquots) at-20 °C.
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- 抗原
- Kanamycin
- Abstract
- Kanamycin 产品
- 物质类
- Chemical
- 背景
- Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a wide variety of infections. Kanamycin is isolated from the bacterium Streptomyces kanamyceticus and its most commonly used form is kanamycin sulfate. Kanamycin is used in molecular biology as a selective agent most commonly to isolate bacteria (e.g., E. coli) which have taken up genes (e.g., of plasmids) coupled to a gene coding for kanamycin resistance (primarily Neomycin phosphotransferase II [NPT II/Neo]). Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin (50-100 μg/mL) containing agar plates or are grown in media containing kanamycin (50-100 μg/mL). Only the bacteria that have successfully taken up the kanamycin resistance gene become resistant and will grow under these conditions. As a powder kanamycin is white to off-white and is soluble in water (50 mg/mL). Mammalian cells and other eukaryotes are screened using G418, a similar aminoglycoside antibiotic, which KanMX confers resistance against. At least one such gene, Atwbc19 is native to a plant species, of comparatively large size and its coded protein acts in a manner which decreases the possibility of horizontal gene transfer from the plant to bacteria, it may be incapable of giving resistance to kanamycin to bacteria even if gene transfer occurs.Synonyms: Kanamycin A, Kantrex
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