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CDKN2C 抗体

CDKN2C 适用: 人 WB, IHC (p), IP 宿主: 小鼠 Monoclonal DCS-118 unconjugated
产品编号 ABIN487307
发货至: 中国
  • 抗原 See all CDKN2C 抗体
    CDKN2C (Cyclin-Dependent Kinase Inhibitor 2C (p18, Inhibits CDK4) (CDKN2C))
    适用
    • 64
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    宿主
    • 59
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    小鼠
    克隆类型
    • 60
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    单克隆
    标记
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    This CDKN2C antibody is un-conjugated
    应用范围
    • 45
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    特异性
    This antibody reacts with Human p18INK4c.
    交叉反应 (详细)
    Species reactivity (tested):Human.
    产品特性
    Synonyms: p18-INK4c, p18-INK6, p18INK6, CDKN6, Cyclin-dependent kinase 4 inhibitor C,Cyclin-dependent kinase 6 inhibitor
    纯化方法
    Protein-A Sepharose Chromatography.
    免疫原
    Bacterially produced His-tagged p18 proteins.
    克隆位点
    DCS-118
    亚型
    IgG2a
    Top Product
    Discover our top product CDKN2C Primary Antibody
  • 应用备注
    Western Blot: 1 μg/mLPositive Control: Saos-2 Cells. Immunoprecipitation: 3 μg/200-300 μL of cell extract. Positive Control: Saos-2 Cells. Immunohistochemistry: 1-5 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Microwave oven: 2 times for 10 minutes each in citrate buffer ( pH 6.5). Positive Control: Tonsil Tissue. Detailed procedure is provided in Protocols.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for the transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with the anti-p18INK4c (DCS-118) monoclonal antibody (1μg/mL) diluted with 1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 HRP-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Control for Western blotting: Saos-2 cells. Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add 3 μg of the anti-p18INK4c (DCS-118) monoclonal antibody into 250 μL of thesupernatant. Mix well and incubate with gentle agitation for 30-120 minutes at 4°C. Add 20μL of 50% Protein A-agarose beads resuspended in the Lysis buffer. Mix well and incubatewith gentle agitation for 60 minutes at 4°C. 4) Wash the beads 3-5 times with ice-cold Lysis buffer (centrifuge the tube at 2,500 x g for10 seconds). 5) Resuspend the beads in 20 μL of Laemmli’s sample buffer, boil for 3-5 minutes, andcentrifuge for 5 minutes. Use 10 μL/lane for the SDS-PAGE analysis. (See SDS-PAGE & Western blotting. )Positive Control for immunoprecipitation: Saos-2 cells.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.2 containing 50 % Glycerol without preservatives.
    储存液
    Without preservative
    储存条件
    -20 °C
    储存方法
    Store the antibody undiluted at -20 °C.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    CDKN2C (Cyclin-Dependent Kinase Inhibitor 2C (p18, Inhibits CDK4) (CDKN2C))
    别名
    CDKN2C / p18INK4c (CDKN2C 产品)
    别名
    CDKN2C antibody, p18 antibody, ink4c antibody, p18-ink4c antibody, INK4C antibody, p18-INK4C antibody, C77269 antibody, INK4c antibody, INK4d antibody, p18INK4c antibody, cyclin dependent kinase inhibitor 2C antibody, cyclin-dependent kinase inhibitor 2C antibody, cyclin-dependent kinase inhibitor 2C (p18, inhibits CDK4) antibody, CDKN2C antibody, cdkn2c antibody, Cdkn2c antibody
    背景
    The INK4 family of proteins consists of four members that block progression from the G1-to-S phase of the cell cycle by inhibiting the activity of Cdk4 and Cdk6. The p18INK4c cyclin-dependent kinase inhibitor is an important regulator of cellular differentiation and cell cycle progression, and it also acts as a potent tumor suppressor. p18INK4c is regulated by the transcription factors E2F1 and SP1 in response to environmental and intracellular signals such as cytokines, oncogenic overload, or cellular senescence.Synonyms: CDKN6, Cyclin-dependent kinase 4 inhibitor C, Cyclin-dependent kinase 6 inhibitor, p18-INK4c, p18-INK6, p18INK6
    基因ID
    1031
    UniProt
    P42773
    途径
    Cell Division Cycle, Mitotic G1-G1/S Phases
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