SSEA-4 抗体
SSEA-4
适用: 人, 小鼠
FACS, IHC, IHC (fro)
宿主: 小鼠
Monoclonal
unconjugated
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- 抗原 See all SSEA-4 products
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SSEA-4 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.
- 纯化方法
- Protein A purified
- 免疫原
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Human embryonal carcinoma cell line 2102 Ep.
Type of Immunogen: Cells - 亚型
- IgG3
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- 应用备注
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Approved: Flo, IHC, IHC-Fr
Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- 抗原
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
- 别名
- SSEA-4 (SSEA-4 产品)
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