NeuN 抗体 (Biotin)
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- 抗原 See all NeuN (RBFOX3) 抗体
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
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适用
- 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NeuN antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Reacts with most neuronal cell types throughout the nervous system of mice including cerebellum, cerebral cortex, hippocampus, thalamus, spinal cord and neurons in the peripheral nervous system including dorsal root ganglia, sympathetic chain ganglia and enteric ganglia. Immunohistochemical staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. The few cell types not reactive include Purkinje, mitral and photoreceptor cells. Species cross-reactivity: Rat. It is expected that it will also react with human, ferret, chick and salamander.
- 纯化方法
- Protein A purified
- 免疫原
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Purified cell nuclei from mouse brain.
Type of Immunogen: Purified protein - 克隆位点
- A60
- 亚型
- IgG1
- Top Product
- Discover our top product RBFOX3 Primary Antibody
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anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibody
RBFOX3 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 1H3B2 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-220) antibodyRBFOX3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-140) antibodyRBFOX3 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5A4B9 unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 51-150) antibodyRBFOX3 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyRBFOX3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) (AA 1-101) antibodyRBFOX3 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3) antibodyMicroarray verified RBFOX3 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB503575 unconjugated
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- 应用备注
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Approved: ICC (1:10 - 1:500), IHC, IHC-P, WB
Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Immunohistochemistry: 1:100-1:2000, Works best on polyester wax embedded tissue but also works on paraffin embedded tissue at a lower working dilution. Works well with formaldehyde-based fixatives. Citric acid and microwave pretreatment has been used successfully. Staining is primarily in the nucleus of the neurons with lighter staining in the cytoplasm. Developmentally, immunoreactivity is first observed shortly after neurons have become postmitotic, no staining has been observed in proliferative zones. Western Blot: Recognizes 2-3 bands in the 46-48kD range and possibly another band at ~66kD. Immunocytochemistry: 1:10-1:500 dilution with buffer without excess protein blocks or detergents. Neurons in culture should be permeabilized with 0.1 % triton X-100. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.1, 0.09 % sodium azide, 15 mg/mL BSA.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- avoid freeze thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- 抗原
- NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))
- 别名
- NeuN (RBFOX3 产品)
- 背景
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Name/Gene ID: NeuN
Synonyms: NeuN
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