Soluble Terminal Complement Complex (sC5b-9) 抗体
sC5b-9
适用: 人
FACS, IHC (fro), WB, ELISA, IHC
宿主: 小鼠
Monoclonal
3R2-0
unconjugated
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- 抗原
- Soluble Terminal Complement Complex (sC5b-9)
- 适用
- 人
-
宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- Recognizes the SC5b-9 complex of around 330 kD. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kD C5b which is bound to cells via 71 kD C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kD S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Removal of Sodium Azide is recommended prior to use in functional assays. Reported to work in western blotting applications.
- 纯化方法
- Affinity purified
- 免疫原
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Type of Immunogen: Native protein - 克隆位点
- 3R2-0
- 亚型
- IgG2b
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Borate buffered saline, pH 8.4, <0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- avoid freeze thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- 抗原
- Soluble Terminal Complement Complex (sC5b-9)
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