IL-33 抗体
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- 抗原 See all IL-33 (IL33) 抗体
- IL-33 (IL33) (Interleukin 33 (IL33))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-33 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Human IL-33
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human IL-33.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL33 Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), SwELISA, WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- IL-33 (IL33) (Interleukin 33 (IL33))
- 别名
- IL33 (IL33 产品)
- 别名
- IL33 antibody, C9orf26 antibody, DVS27 antibody, IL1F11 antibody, NF-HEV antibody, NFEHEV antibody, RP11-575C20.2 antibody, 9230117N10Rik antibody, Il-33 antibody, Il1f11 antibody, RGD1311155 antibody, interleukin 33 antibody, IL33 antibody, Il33 antibody
- 背景
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Name/Gene ID: IL33
Family: Interleukin
Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2 - 基因ID
- 90865
- UniProt
- O95760
- 途径
- Production of Molecular Mediator of Immune Response
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