G-CSF 抗体
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- 抗原 See all G-CSF (CSF3) 抗体
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This G-CSF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut)
- 特异性
- Human G-CSF
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human G-CSF.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CSF3 Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
- 别名
- CSF3 / G-CSF / GCSF (CSF3 产品)
- 别名
- CSF3 antibody, G-CSF antibody, gcsf antibody, Csfg antibody, MGI-IG antibody, C17orf33 antibody, CSF3OS antibody, GCSF antibody, Gcsf antibody, colony stimulating factor 3 antibody, colony stimulating factor 3 (granulocyte) a antibody, colony stimulating factor 3 (granulocyte) antibody, CSF3 antibody, csf3a antibody, Csf3 antibody
- 背景
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Name/Gene ID: CSF3
Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim - 基因ID
- 1440
- UniProt
- P09919
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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