VEGFA 抗体 (Biotin)
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- 抗原 See all VEGFA 抗体
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGFA antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut)
- 特异性
- Rat VEGF
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Rat VEGF.
Type of Immunogen: Cell extract - Top Product
- Discover our top product VEGFA Primary Antibody
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- 应用备注
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Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat VEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. To detect Rat VEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat VEGF (LS-C104547) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat VEGF. Western Blot: To detect Rat VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat VEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Rat
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile PBS containing 0.1 % BSA
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- VEGFA / VEGF (VEGFA 产品)
- 背景
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Name/Gene ID: VEGFA
Family: PDGF
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 - 基因ID
- 7422
- UniProt
- P15692
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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