VEGFA 抗体
-
- 抗原 See all VEGFA 抗体
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This VEGFA antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA
- 特异性
- Human VEGF
- 纯化方法
- Protein A purified
- 免疫原
-
E.coli derived recombinant Human VEGF165.
Type of Immunogen: Cell extract - 亚型
- IgG1
- Top Product
- Discover our top product VEGFA Primary Antibody
-
-
- 应用备注
-
Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/mL), a concentration of &le. 0.2 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 (LS-C104615) as the detection antibody at a concentration of approximately 0.25-1 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- 抗原
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- VEGFA / VEGF (VEGFA 产品)
- 背景
-
Name/Gene ID: VEGFA
Family: PDGF
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 - 基因ID
- 7422
- UniProt
- P15692
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-