CCL5 抗体
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- 抗原 See all CCL5 抗体
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CCL5 antibody is un-conjugated
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应用范围
- Neutralization (Neut)
- 特异性
- Human RANTES
- 纯化方法
- Protein A purified
- 免疫原
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E.coli derived recombinant Human RANTES.
Type of Immunogen: Cell extract - 亚型
- IgG2b
- Top Product
- Discover our top product CCL5 Primary Antibody
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- 应用备注
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Approved: Neut (5 - 7 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
- 别名
- CCL5 / RANTES (CCL5 产品)
- 别名
- Rantes antibody, Scya5 antibody, D17S136E antibody, RANTES antibody, SCYA5 antibody, SIS-delta antibody, SISd antibody, TCP228 antibody, eoCP antibody, MuRantes antibody, C-C motif chemokine 5-like antibody, C-C motif chemokine ligand 5 antibody, chemokine (C-C motif) ligand 5 antibody, LOC100230935 antibody, CCL5 antibody, Ccl5 antibody
- 背景
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Name/Gene ID: CCL5
Family: Intercrine
Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228 - 基因ID
- 6352
- UniProt
- P13501
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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