Interleukin 17a 抗体
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- 抗原 See all Interleukin 17a (IL17A) 抗体
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Interleukin 17a antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Neutralization (Neut)
- 特异性
- Human IL-17A
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human IL-17A.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL17A Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.43 - 0.65 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-17A (50.00 ng/mL), a concentration of 0.43-0.65 μg/mL of this antibody is required. ELISA: To detect hIL-17A by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17A (LS-C104417) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-17A is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute in sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 别名
- IL17A / IL17 (IL17A 产品)
- 别名
- CTLA8 antibody, IL-17 antibody, IL-17A antibody, IL17 antibody, Ctla-8 antibody, Ctla8 antibody, Il17 antibody, ChIL-17 antibody, IL-17F antibody, IL17A antibody, CTLA-8 antibody, interleukin 17A antibody, IL17A antibody, Il17a antibody
- 背景
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Name/Gene ID: IL17A
Family: Interleukin
Synonyms: IL17A, CTLA-8, CTLA8, Interleukin-17A, Interleukin 17A, IL-17, IL-17A, IL17 - 基因ID
- 3605
- UniProt
- Q16552
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