CCL15 抗体
-
- 抗原 See all CCL15 抗体
- CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
- 适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This CCL15 antibody is un-conjugated
-
应用范围
- ELISA, Western Blotting (WB), Neutralization (Neut)
- 特异性
- Human MIP-5
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
E.coli derived recombinant Human MIP-5.
Type of Immunogen: Cell extract
-
-
- 应用备注
-
Approved: ELISA (0.5 - 2 μg/mL), Neut (1.75 - 3 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/mL), a concentration of 1.75-3 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
-
Target Species of Antibody: Human
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- 抗原
- CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))
- 别名
- CCL15 / MIP5 (CCL15 产品)
- 别名
- HCC-2 antibody, HMRP-2B antibody, LKN-1 antibody, LKN1 antibody, MIP-1 delta antibody, MIP-1D antibody, MIP-5 antibody, MRP-2B antibody, NCC-3 antibody, NCC3 antibody, SCYA15 antibody, SCYL3 antibody, SY15 antibody, C-C motif chemokine ligand 15 antibody, CCL15 antibody
- 背景
-
Name/Gene ID: CCL15
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15 - 基因ID
- 6359
- UniProt
- Q16663
-