BRCC3 抗体 (AA 1-316)
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- 抗原 See all BRCC3 抗体
- BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))
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抗原表位
- AA 1-316
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BRCC3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- BRCC36 (1-316aa)
- 纯化方法
- Protein G purified
- 免疫原
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Recombinant BRCC36 (1-316aa) purified from E. coli
Type of Immunogen: Recombinant protein - 克隆位点
- AT3B1
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product BRCC3 Primary Antibody
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- 应用备注
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Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.1 % sodium azide. Sourced in Ascites.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))
- 别名
- BRCC3 / BRCC36 (BRCC3 产品)
- 别名
- CXorf53 antibody, BRCC3 antibody, xtp1 antibody, brcc3 antibody, BRCC36 antibody, brcc36 antibody, C6.1A antibody, BRCA1/BRCA2-containing complex subunit 3 antibody, DEP domain containing 1B antibody, BRCA1/BRCA2-containing complex, subunit 3 antibody, BRCA1/BRCA2-containing complex subunit 3 L homeolog antibody, BRCC3 antibody, depdc1b antibody, brcc3 antibody, Brcc3 antibody, brcc3.L antibody
- 背景
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Name/Gene ID: BRCC3
Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2 - 基因ID
- 79184
- UniProt
- P46736
- 途径
- Positive Regulation of Response to DNA Damage Stimulus
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