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BRCC3 抗体 (AA 1-316)

BRCC3 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal AT3B1 unconjugated
产品编号 ABIN462274
发货至: 中国
  • 抗原 See all BRCC3 抗体
    BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))
    抗原表位
    • 15
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-316
    适用
    • 55
    • 16
    • 15
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    宿主
    • 50
    • 5
    小鼠
    克隆类型
    • 52
    • 3
    单克隆
    标记
    • 26
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This BRCC3 antibody is un-conjugated
    应用范围
    • 41
    • 18
    • 13
    • 13
    • 11
    • 8
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    BRCC36 (1-316aa)
    纯化方法
    Protein G purified
    免疫原
    Recombinant BRCC36 (1-316aa) purified from E. coli

    Type of Immunogen: Recombinant protein
    克隆位点
    AT3B1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product BRCC3 Primary Antibody
  • 应用备注
    Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))
    别名
    BRCC3 / BRCC36 (BRCC3 产品)
    别名
    CXorf53 antibody, BRCC3 antibody, xtp1 antibody, brcc3 antibody, BRCC36 antibody, brcc36 antibody, C6.1A antibody, BRCA1/BRCA2-containing complex subunit 3 antibody, DEP domain containing 1B antibody, BRCA1/BRCA2-containing complex, subunit 3 antibody, BRCA1/BRCA2-containing complex subunit 3 L homeolog antibody, BRCC3 antibody, depdc1b antibody, brcc3 antibody, Brcc3 antibody, brcc3.L antibody
    背景
    Name/Gene ID: BRCC3

    Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2
    基因ID
    79184
    UniProt
    P46736
    途径
    Positive Regulation of Response to DNA Damage Stimulus
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