Ubiquitin 抗体
-
- 抗原 See all Ubiquitin 抗体
- Ubiquitin
-
适用
- 人, 小鼠, Cow
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Ubiquitin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 品牌
- IHC-plus™
- 特异性
- Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.
- 纯化方法
- Protein A purified
- 免疫原
-
Purified bovine ubiquitin conjugated to carrier protein.
Type of Immunogen: Purified protein - 亚型
- IgG1 kappa
- Top Product
- Discover our top product Ubiquitin Primary Antibody
-
anti-Ubiquitin (Ubiquitin) antibody
Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 小鼠, 大鼠, Cow, Pig, 兔, 犬, 马 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) (AA 1-229) antibodyUbiquitin 适用: 人, 大鼠 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Ubiquitin (Ubiquitin) (acLys27) antibodyUbiquitin 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin (Ubiquitin) antibody (HRP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP
anti-Ubiquitin (Ubiquitin) antibody (Alkaline Phosphatase (AP))Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Ubiquitin (Ubiquitin) antibody (FITC)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
anti-Ubiquitin (Ubiquitin) antibody (Biotin)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin
anti-Ubiquitin (Ubiquitin) antibody (PE)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE
anti-Ubiquitin (Ubiquitin) antibody (PerCP)Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PerCP
-
- 应用备注
-
Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl, pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing. - 说明
-
Target Species of Antibody: Bovine
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 0.1 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- 抗原
- Ubiquitin
- 别名
- Ubiquitin (Ubiquitin 产品)
- 途径
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
-
