BNIP3 抗体 (AA 112-124)
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- 抗原 See all BNIP3 抗体
- BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
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抗原表位
- AA 112-124
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This BNIP3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 品牌
- IHC-plus™
- 特异性
- Species cross-reactivity: Human.
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Recombinant fragment (Residues 1-163 of Human BNIP3). Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.
Type of Immunogen: Recombinant protein - 亚型
- IgG2b
- Top Product
- Discover our top product BNIP3 Primary Antibody
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- 应用备注
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Approved: ELISA, IHC, IHC-P (10 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 2-4 μg/mL. Detects a band at ~30kD and ~60kD. Positive control: Whole cell lysate from 293T cells transfected with BNIP3. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, 15 mM sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- 4°C while lyophilized, once reconstituted, aliquot and store at -70°C.
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- 抗原
- BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
- 别名
- NIP3 / BNIP3 (BNIP3 产品)
- 别名
- NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody
- 背景
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Name/Gene ID: BNIP3
Synonyms: BNIP3, NIP3 - 基因ID
- 664
- UniProt
- Q12983
- 途径
- Autophagy, Brown Fat Cell Differentiation
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