GAPDH 抗体 (Biotin)
-
- 抗原 See all GAPDH 抗体
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
-
适用
- 兔
-
宿主
-
绵羊
-
克隆类型
- 多克隆
-
标记
- This GAPDH antibody is conjugated to Biotin
-
应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal sheep antiserum to glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product GAPDH Primary Antibody
-
-
- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.9. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
- 抗原
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- 别名
- Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH 产品)
- 别名
- G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
-