PLC 抗体 (Biotin)
PLC
适用: Bacillus cereus
WB, IF, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all PLC 抗体
- PLC (Phospholipase C (PLC))
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适用
- Bacillus cereus
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PLC antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product PLC Primary Antibody
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.4. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- PLC (Phospholipase C (PLC))
- 别名
- Phospholipase C (PLC 产品)
- 别名
- GB16555 antibody, PLC antibody, ARABIDOPSIS THALIANA PHOSPHOLIPASE C antibody, ATPLC antibody, ATPLC1 antibody, MZN1.12 antibody, MZN1_12 antibody, PHOSPHOLIPASE C antibody, phospholipase C 1 antibody, phospholipase C1 antibody, BA0677 antibody, BCE0744 antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1 antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase classes I and II antibody, phospholipase C antibody, phospholipase C1 antibody, uncharacterized LOC100851276 antibody, plc-1 antibody, LOC408791 antibody, PLC antibody, PLC1 antibody, plc antibody, plC antibody, LOC100851276 antibody, LOC100009319 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
- 途径
- TCR Signaling, Response to Water Deprivation, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Phototransduction
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