NADH Peroxidase 抗体 (Biotin)
适用: Enterococcus faecalis
WB, IF, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all NADH Peroxidase products
- NADH Peroxidase
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适用
- Enterococcus faecalis
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NADH Peroxidase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to NADH peroxidase from Streptococcus faecalis
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- NADH peroxidase isolated and purified from Streptococcus faecalis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.4. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- NADH Peroxidase
- Abstract
- NADH Peroxidase 产品
- 别名
- putative NADH peroxidase antibody, NADH peroxidase antibody, NAD(FAD)-dependent dehydrogenase antibody, SPy_1681 antibody, spyM18_1693 antibody, npx antibody, SPs0401 antibody, npr antibody, MA_RS07405 antibody, M6_Spy1426 antibody, M5005_Spy_1378 antibody, M28_Spy1421 antibody, MBAR_RS14975 antibody, LSL_0137 antibody, MBUR_RS05050 antibody, SpyM50412 antibody, LCRIS_RS02695 antibody, Lbuc_1777 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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