LDH5 抗体 (Biotin)
LDH5
适用: Pig
WB, IF, ELISA
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all LDH5 products
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
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适用
- Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LDH5 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), ELISA
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to L-lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, from porcine heart
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- L-Lactic dehydrogenase, LDH-1(M 4 ) isoenzyme, isolated and purified from porcine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.4. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- LDH5 (Lactate Dehydrogenase Isoenzyme 5 (LDH5))
- Abstract
- LDH5 产品
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immuno-diffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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