Choline Oxidase 抗体 (Biotin)
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- 抗原 See all Choline Oxidase products
- Choline Oxidase
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适用
- Arthrobacter globiformis
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Choline Oxidase antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to choline oxidase from Athrobacter globiformis
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- Choline oxidase isolated and purified from Athrobacter globiformis. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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anti-Choline Oxidase antibody (HRP)
适用: Alcaligenes WB, ELISA, IP 宿主: 山羊 Polyclonal HRP
anti-Choline Oxidase antibody (Biotin)适用: Alcaligenes WB, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Choline Oxidase antibody适用: Alcaligenes ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 5.6. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- Choline Oxidase
- Abstract
- Choline Oxidase 产品
- 别名
- Afu8g04090 antibody, choline oxidase antibody, choline oxidase (CodA) antibody, SAV_6946 antibody, AFUA_8G04090 antibody, AAur_0512 antibody, Bfae_22540 antibody, BDBG_07023 antibody, codA antibody, Mspyr1_24000 antibody, TERG_03224 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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