Carbonic Anhydrase 抗体 (Biotin)
CA
适用: Cow
ELISA, WB, IF
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all Carbonic Anhydrase (CA) products
- Carbonic Anhydrase (CA)
- 适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Carbonic Anhydrase antibody is conjugated to Biotin
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应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to carbonic anhydrase from bovine erythrocytes
- 纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 免疫原
- Carbonic anhydrase isolated and purified from bovine erythrocytes. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Biotin/ IgG protein molar ratio (B/P) approximately 4.9. No foreign proteins added.
- 缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- 抗原
- Carbonic Anhydrase (CA)
- 别名
- Carbonic Anhydrase (CA 产品)
- 别名
- ca2 antibody, AO090010000582 antibody, alpha carbonic anhydrase 5 antibody, ALPHA CARBONIC ANHYDRASE 5 antibody, ATACA5 antibody, T6D22.30 antibody, T6D22_30 antibody, Carbonic AnHydrase antibody, carbonic anhydrase antibody, alpha carbonic anhydrase 5 antibody, interferon regulatory factor 2-binding protein 1 antibody, cah-6 antibody, ca2 antibody, CA antibody, AOR_1_970024 antibody, ACA5 antibody, LOC790100 antibody
- 背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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