HRP 抗体
HRP
适用: Horseradish
WB, ELISA
宿主: 小鼠
Monoclonal
NI 91
unconjugated
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- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HRP antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- This antiserum has not been tested for cross-reactivity.
- 产品特性
- Purified monoclonal mouse antibody to horseradish peroxidase
- 纯化方法
- Purified
- 免疫原
- Highly purified horseradish peroxidase
- 克隆位点
- NI 91
- 亚型
- IgG1 kappa
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish WB, ELISA, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
- TAs a control in the tests where mouse IgG1 (with irrelevant specificity) should be used to show the presence of that given mouse isotype. In indirect tests as reagent to horseradish peroxidase or to potentiatethe visualisation of a peroxidase labelled conjugate in the appropriate material. As secondary reagent a labelled anti mouse IgG will serve for the detection. This system can be further be potentiated by the use of anti-FITC or anti-TRITC conjugates. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG concentration is 0.4 mg/ml. No foreign proteins added.
- 缓冲液
- Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- Horseradish Peroxidase (HRP 产品)
- 背景
- The reactivity of the antiserum is directed to horseradish as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
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