Homologous proteins of different species frequently share antigenic determinants. has not been tested.
产品特性
IgG fraction of polyclonal rabbit antiserum to sheep erythrocytes
纯化方法
Adsorption: No adsorption required. Cross-reactivity Homologous proteins of different species frequently share antigenic determinants. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
免疫原
Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.
To sensitize cells in haemolytic complement assays and for screening of T and T receptor-bearing lymphocytes.
限制
仅限研究用
状态
Lyophilized
浓度
IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Haemolytic titre, at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig complement (at a 1:60 dilution) and 5x10 8 RBC/ml, not less than 1:3,000
缓冲液
Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
储存液
Without preservative
储存条件
4 °C/-20 °C
储存方法
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.