(2 references)-
- 抗原 See all Fibrinogen 抗体
- Fibrinogen
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Fibrinogen antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.
- 产品特性
- Purified IgG fraction of polyclonal goat antiserum to mouse fibrinogen
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
- 免疫原
- Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product Fibrinogen Primary Antibody
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anti-Fibrinogen antibody
适用: 人, 大鼠 ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen antibody (Biotin)适用: 人 ELISA, WB, IHC 宿主: 山羊 Polyclonal Biotin
anti-Fibrinogen antibody适用: 人 IHC (p), IF (p) 宿主: 小鼠 Monoclonal 4F7 unconjugated
anti-Fibrinogen antibody适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Fibrinogen antibody适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
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- 应用备注
- As unlabelled primary or secondary antibody reagent for the indirect detection of fibrinogen in mouse cells, tissues and body fluids in immunofluorescence and immunoenzyme methods, for the production of immunoconjugates with a selected marker, to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier, as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added.
- 缓冲液
- Purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
- 储存液
- Without preservative
- 储存条件
- 4 °C
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: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." in: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).
: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." in: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).
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: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." in: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).
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- 抗原
- Fibrinogen
- Abstract
- Fibrinogen 产品
- 别名
- fib2 antibody, MGC53620 antibody, fibrinogen antibody, sb:cb892 antibody, wu:fa55c12 antibody, wu:fb20e08 antibody, wu:fb59b03 antibody, wu:fi39d04 antibody, zgc:92117 antibody, fibrinogen alpha chain antibody, fibrinogen gamma chain antibody, fga antibody, FGA antibody, LOC698244 antibody, CpipJ_CPIJ010090 antibody, FGG antibody
- 背景
- The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products
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