Kininogen (HMW) 抗体
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- 抗原 See all Kininogen (HMW) 抗体
- Kininogen (HMW) (Kininogen (High Molecular Weight))
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Kininogen (HMW) antibody is un-conjugated
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应用范围
- Immunodiffusion (ID), Immunoelectrophoresis (IEP)
- 特异性
- The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with monkey has been observed.
- 产品特性
- Precipitating polyclonal goat antiserum to human high molecular weight kininogen
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
- 免疫原
- Highly purified molecular weight kininogen isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product Kininogen (HMW) Primary Antibody
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anti-Kininogen (High Molecular Weight) (AA 225-407) antibody
适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kininogen (High Molecular Weight) (AA 214-475) antibody适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kininogen (High Molecular Weight) (AA 268-540) antibody适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kininogen (High Molecular Weight) (AA 58-176) antibody适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- As precipitating antiserum in electroimmunodiffusion, immunoelectrophoresis and radial immunodiffusion. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human plasma in agar block immunodiffusion
- 缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole serum.
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same da y.
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- 抗原
- Kininogen (HMW) (Kininogen (High Molecular Weight))
- Abstract
- Kininogen (HMW) 产品
- 别名
- Kng antibody, kininogen 1 antibody, Kng1 antibody
- 背景
- The defined antibody specificity is to human high molecular weight kininogen. In immunoelectrophoresis, bi-dimensional electrophoresis and radial immunodiffusion (Ouchterlony, Mancini), using various antiserum concentrations against normal plasma, one single precipitin line is obtained. No precipitation is observed against plasma of patients with high molecular weight kininogen deficiency, but its presence can be demonstrated in normal serum. In double radial immunodiffusion the precipitin line obtained against normal plasma shows a reaction of full identity with the precipitin line with purified high molecular weight kininogen
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