Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results: bovine + cat + chicken - dog + duck - goat + guinea pig + hamster ++ human + monkey ++ mouse + pigeon - rabbit ± rat + sheep + swine + A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
产品特性
Precipitating polyclonal rabbit antiserum to horse serum albumin
纯化方法
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
免疫原
Highly purified albumin isolated from horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
As precipitating antiserum to identify or measure horse albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
限制
仅限研究用
状态
Lyophilized
浓度
Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal horse serum in agar block immunodiffusion titra
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonge d storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
The defined antibody specificity is directed to albumin as tested against horse sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against horse serum and the purified albumin.