LPO 抗体 (Biotin)
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- 抗原 See all LPO 抗体
- LPO (Lactoperoxidase (LPO))
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适用
- Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LPO antibody is conjugated to Biotin
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应用范围
- Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA
- 特异性
- The antiserum does not cross-react with any other component of bovine serum. Inter-species cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail, but reactivity with lactoperoxidase from goat and sheep may be expected.
- 产品特性
- Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to bovine lactoperoxidase
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the anti-serum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- 免疫原
- Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It sis isolated from pooled bovine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product LPO Primary Antibody
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anti-Lactoperoxidase (LPO) (AA 351-589) antibody
LPO 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 305-498) antibodyLPO 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 244-346) antibodyLPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lactoperoxidase (LPO) (AA 351-589) antibodyLPO 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 15# unconjugated
anti-Lactoperoxidase (LPO) antibody (Biotin)LPO 适用: Cow WB, IP, ELISA 宿主: 绵羊 Polyclonal Biotin
anti-Lactoperoxidase (LPO) antibody (HRP)LPO 适用: Cow WB, ELISA 宿主: 绵羊 Polyclonal HRP
anti-Lactoperoxidase (LPO) antibodyLPO 适用: Cow ELISA 宿主: 绵羊 Polyclonal unconjugated
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- 应用备注
- In immunocytochemical and immunohistochemical staining to identify and measure lactoperoxidase at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. Working dilutions for histochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:5,000.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- Physicochemical characteristic IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 8.9. No foreign proteins added.
- 缓冲液
- Biotin-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
- 储存液
- Without preservative
- 储存条件
- 4 °C/-20 °C
- 储存方法
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- 抗原
- LPO (Lactoperoxidase (LPO))
- 别名
- Lactoperoxidase (LPO 产品)
- 别名
- LPO antibody, SPO antibody, 5830499B15Rik antibody, lactoperoxidase antibody, LPO antibody, Lpo antibody
- 背景
- The reactivity of the antiserum is restricted to lactoperoxidase as tested in immuno-electrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma
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