我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Ly6g 抗体

Name: Ly6g 抗体
Brand: antibodies-online
SKU: ABIN457407
Availability: OutOfStock

Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin, concentration in sample 9 μg/mL) DAR PE. (Ly6g 抗体)

FACS

Separation of murine Ly6G positive cells (red-filled) from Ly6G negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse Ly6G (RB6-8C5) purified antibody (low endotoxin, concentration in sample 9 μg/mL) DAR PE. (Ly6g 抗体)

FACS

2 images

(8 references)

产品编号 ABIN457407

发货至: 中国

Datasheet as PDF

抗原 See all Ly6g products

Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
适用
84

13

1
小鼠
宿主
64

19

1
大鼠
Compatible Secondaries
克隆类型
64

18

1
单克隆
标记
21

9

8

6

5

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Ly6g antibody is un-conjugated
应用范围
62

33

21

20

20

14

11

8

8

7

4

3

2

2

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

特异性

The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

交叉反应（详细）

Mouse

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

内毒素水平

Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

免疫原

Murine granulocytes

克隆位点

RB6-8C5

亚型

IgG2b
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (APC)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE-Cy7)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 FITC

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (Biotin)
Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 Biotin

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (violetFluor™ 450)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 violetFluor™ 450

应用备注

Functional application: Neutrophil depletion.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein.
Western blotting: Recommended dilution: 1 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Jung, Zindl, Lai, Weaver, Chaplin: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).

dos Santos, Vaz Cardoso, Nascimento, Lino, Dorta, de Oliveira, Ribeiro-Dias: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." in: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, (2008) (PubMed).

Leendertse, Willems, Giebelen, Roelofs, Bonten, van der Poll: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." in: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, (2008) (PubMed).

Fan, Li, Levy, Fan, Hackam, Vodovotz, Yang, Tracey, Billiar, Wilson: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, (2007) (PubMed).

Koo, Gan: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." in: BMC immunology, Vol. 7, pp. 19, (2006) (PubMed).

Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Brown, Blaho, Loiacono: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, (2003) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).
抗原

Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

别名

Ly6G (Ly6g 产品)

别名

Gr-1 antibody, Gr1 antibody, Ly-6G antibody, lymphocyte antigen 6 complex, locus G antibody, Ly6g antibody

背景

Lymphocyte antigen 6 complex, locus G,Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.,Gr1, Gr-1, Ly-6G

基因ID

546644

UniProt

A0A087WQF5