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CD3 抗体

This anti-CD3 antibody is a 小鼠 单克隆 antibody detecting CD3 in FACS, IHC (fro) 和 Func. Suitable for 人. This Primary Antibody has been cited in 9+ publications.
Pubmed (9 references)
产品编号 ABIN457398
发货至: 中国

Quick Overview for CD3 抗体 (ABIN457398)

抗原

See all CD3 抗体
CD3

适用

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人

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD3 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

克隆位点

OKT3
Discover our top product CD3 Primary Antibody

  • 原理

    Anti-Hu CD3 Purified Low Endotoxin

    特异性

    The mouse monoclonal antibody OKT3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody, also known as Orthoclone OKT3 or Muromonab-CD3, has been extensively used as a drug for therapy of acute, glucocorticoid resistant rejection of allogenic renal, heart and liver transplants. It has also been investigated for use in treating T-cell acute lymphoblastic leukemia.

    交叉反应 （详细）

    Human

    纯化方法

    Purified by protein-A affinity chromatography.

    纯度

    > 95 % (by SDS-PAGE)

    内毒素水平

    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

    免疫原

    human T cells

    亚型

    IgG2a
  • anti-CD3 (CD3) antibody
    anti-CD3 (CD3) antibody

    CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated

    anti-CD3 (CD3) antibody
    anti-CD3 (CD3) antibody

    CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated

    anti-CD3 (CD3) antibody
    anti-CD3 (CD3) antibody

    CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated

    anti-CD3 (CD3) antibody
    anti-CD3 (CD3) antibody

    CD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated

    anti-CD3 (CD3) antibody
    anti-CD3 (CD3) antibody

    CD3 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated

    anti-CD3 (CD3) antibody (Biotin)
    anti-CD3 (CD3) antibody (Biotin)

    CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin

    anti-CD3 (CD3) antibody (Biotin)
    anti-CD3 (CD3) antibody (Biotin)

    CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin

    anti-CD3 (CD3) antibody (PerCP)
    anti-CD3 (CD3) antibody (PerCP)

    CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP

    anti-CD3 (CD3) antibody (APC)
    anti-CD3 (CD3) antibody (APC)

    CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC

    anti-CD3 (CD3) antibody (PE)
    anti-CD3 (CD3) antibody (PE)

    CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE

  • 应用备注

    Functional application: counteracting both generation and function of effector T cells.
    Flow cytometry: Recommended dilution: 1 μg/mL.

    限制

    仅限研究用

  • 浓度

    1 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.4

    注意事项

    Do not freeze.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Do not freeze.
  • Southworth, Plumb, Gupta, Pearson, Ramis, Lehner, Miralpeix, Singh: "Anti-inflammatory potential of PI3Kδ and JAK inhibitors in asthma patients." in: Respiratory research, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

    Li, Wong, Yi, Wong, Zhou, Liu et al.: "Matrine induces cell anergy in human Jurkat T cells through modulation of mitogen-activated protein kinases and nuclear factor of activated T-cells signaling with concomitant up-regulation of ..." in: Biological & pharmaceutical bulletin, Vol. 33, Issue 1, pp. 40-6, (2010) (PubMed).

    Quintana, Schwindling, Wenning, Becherer, Rettig, Schwarz, Hoth: "T cell activation requires mitochondrial translocation to the immunological synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 36, pp. 14418-23, (2007) (PubMed).

    Trushin, Bren, Asin, Pennington, Paya, Badley: "Human immunodeficiency virus reactivation by phorbol esters or T-cell receptor ligation requires both PKCalpha and PKCtheta." in: Journal of virology, Vol. 79, Issue 15, pp. 9821-30, (2005) (PubMed).

    Undale, van den Elsen, Celis: "Antigen-independent acquisition of MHC class II molecules by human T lymphocytes." in: International immunology, Vol. 16, Issue 10, pp. 1523-33, (2004) (PubMed).

    Lang, Handgretinger, Niethammer, Schlegel, Schumm, Greil, Bader, Engel, Scheel-Walter, Eyrich, Klingebiel: "Transplantation of highly purified CD34+ progenitor cells from unrelated donors in pediatric leukemia." in: Blood, Vol. 101, Issue 4, pp. 1630-6, (2003) (PubMed).

    Gordon, Leimig, Mueller, Babarin-Dorner, Holladay, Houston, Kerst, Geiger, Handgretinger: "A large-scale method for T cell depletion: towards graft engineering of mobilized peripheral blood stem cells." in: Bone marrow transplantation, Vol. 30, Issue 2, pp. 69-74, (2002) (PubMed).

    Schlegel, Eyrich, Bader, Handgretinger, Lang, Niethammer, Klingebiel: "OKT-3-based reconditioning regimen for early graft failure in HLA-non-identical stem cell transplants." in: British journal of haematology, Vol. 111, Issue 2, pp. 668-73, (2001) (PubMed).

    Smith: "Ten years of Orthoclone OKT3 (muromonab-CD3): a review." in: Journal of transplant coordination : official publication of the North American Transplant Coordinators Organization (NATCO), Vol. 6, Issue 3, pp. 109-19; quiz 120-1, (1997) (PubMed).

  • 抗原

    CD3

    别名

    CD3

    背景

    CD3 antigen, epsilon polypeptide,CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.,CD3E, T3E, TCRE

    基因ID

    916

    UniProt

    P07766

    途径

    TCR Signaling, Ubiquitin Proteasome Pathway
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