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Ly6g 抗体 (FITC)

Name: Ly6g 抗体 (FITC)
Brand: antibodies-online
SKU: ABIN457353
Availability: OutOfStock

Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 FITC

Flow cytometry surface staining pattern of murine bone marrow cells stained using anti-mouse Ly6G (RB6-8C5) FITC antibody (concentration in sample 0,5 μg/mL). (Ly6g 抗体 (FITC))

FACS

Surface staining of murine splenocytes with anti-Ly-6G (RB6-8C5) FITC. (Ly6g 抗体 (FITC))

FACS

Separation of murine Ly6G positive cells (red-filled) from murine Ly6G negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine bone marrow cells stained using anti-mouse Ly6G (RB6-8C5) FITC antibody (concentration in sample 0,5 μg/mL). (Ly6g 抗体 (FITC))

FACS

3 images

(8 references)

产品编号 ABIN457353

发货至: 中国

Datasheet as PDF

抗原 See all Ly6g products

Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))
适用
84

13

1
小鼠
宿主
64

19

1
大鼠
Compatible Secondaries
克隆类型
64

18

1
单克隆
标记
22

9

7

6

5

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Ly6g antibody is conjugated to FITC
应用范围
62

34

21

21

21

15

11

9

8

7

4

3

2

2

1
Flow Cytometry (FACS)

特异性

The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

交叉反应（详细）

Mouse

纯化方法

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

免疫原

Murine granulocytes

克隆位点

RB6-8C5

亚型

IgG2b
anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p), Func 宿主: 大鼠 Monoclonal RB6-8C5 unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (APC)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 APC

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE-Cy7)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE-Cy7

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (FITC)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 1A8 FITC

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody
Ly6g 适用: 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (Biotin)
Ly6g 适用: 小鼠 FACS, WB, IP, IHC (fro), IHC (p) 宿主: 大鼠 Monoclonal RB6-8C5 Biotin

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (PE)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 PE

anti-Lymphocyte Antigen 6 Complex, Locus G (Ly6g) antibody (violetFluor™ 450)
Ly6g 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal RB6-8C5 violetFluor™ 450

应用备注

Flow cytometry: Recommended dilution: 1-4 μg/mL.

说明

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.
Avoid prolonged exposure to light.

储存条件

4 °C

储存方法

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Jung, Zindl, Lai, Weaver, Chaplin: "MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways." in: European journal of immunology, Vol. 39, Issue 8, pp. 2281-92, (2009) (PubMed).

dos Santos, Vaz Cardoso, Nascimento, Lino, Dorta, de Oliveira, Ribeiro-Dias: "Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production." in: Experimental parasitology, Vol. 119, Issue 3, pp. 403-10, (2008) (PubMed).

Leendertse, Willems, Giebelen, Roelofs, Bonten, van der Poll: "Neutrophils are essential for rapid clearance of Enterococcus faecium in mice." in: Infection and immunity, Vol. 77, Issue 1, pp. 485-91, (2008) (PubMed).

Fan, Li, Levy, Fan, Hackam, Vodovotz, Yang, Tracey, Billiar, Wilson: "Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 10, pp. 6573-80, (2007) (PubMed).

Koo, Gan: "The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection." in: BMC immunology, Vol. 7, pp. 19, (2006) (PubMed).

Nagendra, Schlueter: "Absence of cross-reactivity between murine Ly-6C and Ly-6G." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 58, Issue 2, pp. 195-200, (2004) (PubMed).

Brown, Blaho, Loiacono: "Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 2, pp. 893-901, (2003) (PubMed).

Hestdal, Ruscetti, Ihle, Jacobsen, Dubois, Kopp, Longo, Keller: "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 1, pp. 22-8, (1991) (PubMed).
抗原

Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

别名

Ly6G (Ly6g 产品)

别名

Gr-1 antibody, Gr1 antibody, Ly-6G antibody, lymphocyte antigen 6 complex, locus G antibody, Ly6g antibody

背景

Lymphocyte antigen 6 complex, locus G,Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.,Gr1, Gr-1, Ly-6G

基因ID

546644

UniProt

A0A087WQF5