(8 references)-
- 抗原 See all MHC Class II (MHC2) 抗体
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This MHC Class II antibody is conjugated to FITC
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
- 交叉反应 （详细）
- Mouse
- 纯化方法
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- Activated C57BL/6 mouse spleen cells
- 克隆位点
- M5-114
- 亚型
- IgG2b
- Top Product
- Discover our top product MHC2 Primary Antibody
-
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p), Func 宿主: 大鼠 Monoclonal M5-114 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibodyMHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC
anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (SPRD)MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 SPRD
-
- 应用备注
- Flow cytometry: Recommended dilution: 2-3 μg/mL.
- 说明
-
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
-
: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).
: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).
: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." in: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).
: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." in: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).
: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." in: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).
: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).
: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
-
: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).
-
- 抗原
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- 别名
- MHC Class II (MHC2 产品)
- 别名
- H-2 antibody, MHC-II antibody, histocompatibility-2, MHC antibody, H2 antibody
- 背景
- MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
-
You are here:
