HCV NS4 抗体
HCV NS4
适用: 人
WB, EIA
宿主: 小鼠
Monoclonal
7B7-5G9
unconjugated
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- 抗原 See all HCV NS4 products
- HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HCV NS4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: Hepatitis C Virus, Non-structural protein NS4A, NS4B
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Synthetic polypeptide in which overlapping sequence of at least 90 residues in lengthwere selected from immunodominant NS-4 region of Chinese HCV strains.
- 克隆位点
- 7B7-5G9
- 亚型
- IgG1
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- 应用备注
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ELISA: React with Human Hepatitis C Virus. Western Blot: Use of HCV NS4 antibody (clone 7B7/5G9) at 0.5 μg/mL will allowvisualization of 0.5-10 μg/lane of synthetic NS-4 peptide and 0.1 μg/lane of RecombinantChimeric HCV polyprotein. Testing is under both reducing and non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
- 缓冲液
- 0.01 M PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- HCV NS4 (Hepatitis C Virus NS4 (HCV NS4))
- 别名
- HCV NS4 (HCV NS4 产品)
- 物质类
- Viral Protein
- 背景
- NS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27- kDa protein. The 4A protein has a molecular weight of 6 kDa.Synonyms: Hepatitis C Virus, NS4B, Non-structural protein NS4A
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