HCV NS3 抗体
HCV NS3
适用: 人
WB, EIA
宿主: 小鼠
Monoclonal
281
unconjugated
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- 抗原 See all HCV NS3 抗体
- HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HCV NS3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: Hepatitis C Virus Serine proteiase NS3
- 纯化方法
- Affinity Chromatography on Protein G.
- 免疫原
- Purified Recombinant Chimeric HCV Polyprotein (555 a. a).
- 克隆位点
- 281
- 亚型
- IgG1
- Top Product
- Discover our top product HCV NS3 Primary Antibody
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- 应用备注
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ELISA. Western Blot: Use of Hepatitis C Virus NS3 antibody (clone 281) at 0.1-1 μg/mL will allowvisualization of 100 ng/lane of both Recombinant Chimeric HCV Polyprotein andRecombinant NS-3 Protein. No reaction can be seen with Synthetic NS-3. Testing is underreducing and non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL
- 缓冲液
- 0.01 M PBS, pH 7.2 without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- HCV NS3 (Hepatitis C Virus NS3 (HCV NS3))
- 别名
- HCV NS3 (HCV NS3 产品)
- 物质类
- Viral Protein
- 背景
- HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins, because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.Synonyms: Hepatitis C Virus Serine proteiase NS3
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