Recombinant Blue Fluorescent Protein 抗体 (Atto 488)
BFP
适用: Entacmaea quadricolor
FACS, ICC, IF
宿主: Alpaca
Monoclonal
1H7
Atto 488
single-domain Antibody (sdAb)
Recombinant Antibody
1 image
-
- 抗原 See all Blue Fluorescent Protein (BFP) products
- Blue Fluorescent Protein (BFP)
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Entacmaea quadricolor
-
宿主
-
Alpaca
- Expression System
- E.coli
-
克隆类型
- 单克隆
-
标记
- This Blue Fluorescent Protein antibody is conjugated to Atto 488
- 应用范围
- Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
- 原理
- Camelid sdAb anti-TagFP conjugated with Atto488, Clone 1H7
- 特异性
- Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
- 交叉反应 （详细）
- Does not cross-react with common GFP- or dsRed derivatives.
- 产品特性
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- 纯化方法
- Produced in: E.coli
- 免疫原
- BFP
- 克隆位点
- 1H7
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
-
-
- 应用备注
- Recommended dilution 1:500
- 说明
-
Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 浓度
- 5 μM
- 缓冲液
- 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light!
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- 有效期
- 12 months
-
- 抗原
- Blue Fluorescent Protein (BFP)
- 别名
- TagBFP (BFP 产品)
- 分子量
- 26 kDa
-
You are here:
