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Recombinant Blue Fluorescent Protein 抗体 (Atto 488)

BFP 适用: Entacmaea quadricolor FACS, ICC, IF 宿主: Alpaca Monoclonal 1H7 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
产品编号 ABIN4368347
发货至: 中国
  • 抗原 See all Blue Fluorescent Protein (BFP) products
    Blue Fluorescent Protein (BFP)
    抗体类型
    Recombinant Antibody
    片段
    single-domain Antibody (sdAb)
    适用
    Entacmaea quadricolor
    宿主
    • 5
    Alpaca
    Expression System
    E.coli
    克隆类型
    • 5
    单克隆
    标记
    • 2
    • 2
    • 1
    This Blue Fluorescent Protein antibody is conjugated to Atto 488
    应用范围
    Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
    原理
    Camelid sdAb anti-TagFP conjugated with Atto488, Clone 1H7
    特异性
    Recognizes mTagBFP, mKate, mKate2, mTagRFP, mTagRFP657 and most common fluorescent proteins deriving from Entacmaea quadricolor
    交叉反应 (详细)
    Does not cross-react with common GFP- or dsRed derivatives.
    产品特性
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    纯化方法
    Produced in: E.coli
    免疫原
    BFP
    克隆位点
    1H7
    Labeling Ratio
    a single fluorophore is coupled to exactly one sdAb
  • 应用备注
    Recommended dilution 1:500
    说明

    Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    5 μM
    缓冲液
    5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light!
    储存条件
    -20 °C,-80 °C
    储存方法
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    有效期
    12 months
  • 抗原
    Blue Fluorescent Protein (BFP)
    别名
    TagBFP (BFP 产品)
    分子量
    26 kDa
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