GFP 抗体 (Texas Red (TR))
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- 抗原 See all GFP 抗体
- GFP (Green Fluorescent Protein (GFP))
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适用
- Aequorea victoria
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GFP antibody is conjugated to Texas Red (TR)
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody reacts to Green Fluorescent Protein (GFP).
- 产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 3.0 moles Texas Red(TM) per mole of Mouse IgG - 纯化方法
- Affinity chromatography on Protein A
- 免疫原
- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
- 克隆位点
- 9F9-F9
- 亚型
- IgG2a
- Top Product
- Discover our top product GFP Primary Antibody
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- 应用备注
- ELISA. Western Blot. Immunohistochemistry.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm where ε0.1% = 0.71)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution stable for a short term at 2-8 °C, but best stored at -20 °C. Following reconstitution store the antibody at -20 °C. Do not aliquot in less than 100 μl.
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- 抗原
- GFP (Green Fluorescent Protein (GFP))
- 别名
- GFP (GFP 产品)
- 背景
- Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
- 基因ID
- 6100
- UniProt
- P42212
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