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- 抗原 See all CD20 (MS4A1) 抗体
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This CD20 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- CQDQESSPIE NDSSP
- 特异性
- This antibody recognizes CD20/MS4A1.
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence from the C Terminus of the protein sequence according to NP_068769.2, NP_690605.1. Genename: MS4A1
- Top Product
- Discover our top product MS4A1 Primary Antibody
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- 应用备注
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Peptide ELISA: 1/8000. Western Blot: 3-5 μg/mL at Overnight incubation. Approx 37 kDa band seen in Daudi celllysates. Immunohistochemistry on Paraffin Sections: 2-5 μg/mL. In paraffin embedded Human Spleen, this antibody shows strong membranous staining inthe the follicles formed by B-cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preserative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1700-10, (2016) (PubMed).
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Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 196, Issue 4, pp. 1700-10, (2016) (PubMed).
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- 抗原
- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
- 别名
- CD20 (MS4A1 产品)
- 别名
- B1 antibody, Bp35 antibody, CD20 antibody, CVID5 antibody, LEU-16 antibody, MS4A2 antibody, S7 antibody, AA960661 antibody, Cd20 antibody, Ly-44 antibody, Ms4a2 antibody, MS4A1 antibody, bp35 antibody, cd20 antibody, ms4a2 antibody, leu-16 antibody, ms4a4c antibody, cd20-like antibody, membrane spanning 4-domains A1 antibody, membrane-spanning 4-domains, subfamily A, member 1 antibody, B-lymphocyte antigen CD20 antibody, MS4A1 antibody, Ms4a1 antibody, ms4a1 antibody, LOC101694281 antibody
- 背景
- CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 Molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1
- 基因ID
- 931
- NCBI登录号
- NP_068769
- UniProt
- P11836
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