Calnexin 抗体 (Internal Region)
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- 抗原 See all Calnexin (CANX) 抗体
- Calnexin (CANX)
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠, 犬, Cow, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Calnexin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- c-SKTPELNLDQ FHDKT
- 特异性
- This antibody recognizes Calnexin.
- 交叉反应 (详细)
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Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX
- Top Product
- Discover our top product CANX Primary Antibody
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- 应用备注
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Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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Inhibition of matrix metalloproteinase activity reverses corneal endothelial-mesenchymal transition." in: The American journal of pathology, Vol. 185, Issue 8, pp. 2158-67, (2016) (PubMed).
: "Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein." in: The Journal of biological chemistry, Vol. 289, Issue 35, pp. 24397-416, (2015) (PubMed).
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Inhibition of matrix metalloproteinase activity reverses corneal endothelial-mesenchymal transition." in: The American journal of pathology, Vol. 185, Issue 8, pp. 2158-67, (2016) (PubMed).
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- 抗原
- Calnexin (CANX)
- 别名
- Calnexin (CANX 产品)
- 别名
- CNX antibody, IP90 antibody, P90 antibody, 1110069N15Rik antibody, AI988026 antibody, Cnx antibody, D11Ertd153e antibody, canx antibody, clgn antibody, clnx antibody, cnx antibody, PP90 antibody, zgc:63524 antibody, wu:fe06b12 antibody, CANX antibody, calnexin antibody, CalNeXin antibody, calnexin L homeolog antibody, CANX antibody, Canx antibody, cnx-1 antibody, canx.L antibody, canx antibody, LOC100283947 antibody
- 背景
- Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
- 基因ID
- 821
- NCBI登录号
- NP_001737
- UniProt
- P27824
- 途径
- MAPK Pathway, Thyroid Hormone Synthesis
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