Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
特异性
This antibody detects endogenous levels of eIF4B only when phosphorylated at Serine 422.
纯化方法
Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
免疫原
Synthetic phosphopeptide derived from Human eIF4B around the phosphorylation site of Serine 422 (T-G-Sp-E-S).
Western blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).Synonyms: Eukaryotic translation initiation factor 4B, eIF-4B