Absorption / Emission: 550 nm / 570 nm Molar Ratio: 2.0 Moles TRITC per mole of Goat IgG
纯化方法
Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
Suitable for highly specific immunological methods requiring extremely low backgroundlevels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow cytometry: 1: 2,000 - 1: 10,000. Immunoflourescence: 1: 500 - 1: 2,500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1.0 mL of deionized water (or equivalent).
浓度
1.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 ° C prior to restoration. Following restoration product can be stored undiluted at 2-8 ° for up to one month or (in aliquots) at -20 °C or below.