FITC 抗体 (Alkaline Phosphatase (AP))
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- 抗原 See all FITC products
- FITC (Fluorescein Isothiocyanate (FITC))
- 适用
- 请咨询
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This FITC antibody is conjugated to Alkaline Phosphatase (AP)
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)
- 纯化方法
- Prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
- Fluorescein conjugated to Goat IgG
- 亚型
- IgG
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- 应用备注
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ELISA: 1: 2,000 - 1: 12,000. Western blot: 1: 500 - 1: 2,500. Dot blot. Immunohistochemistry on frozen sections: 1: 200 - 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA), IgG and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- DO NOT FREEZE!!
- 储存条件
- 4 °C
- 储存方法
- Store the antibody prior and following reconstitution (undiluted) at 2 - 8 °C.
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- 抗原
- FITC (Fluorescein Isothiocyanate (FITC))
- 别名
- FITC (FITC 产品)
- 物质类
- Chemical
- 背景
- Synonyms: Fluorescein Isothiocyanate
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