FIV P24 Gag (Internal Region) 抗体
适用: Cat
WB, EIA, IF
宿主: 兔
Polyclonal
unconjugated
1 image
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- 抗原
- FIV P24 Gag
- 抗原表位
- Internal Region
- 适用
- Cat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)
- 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to amino acids from an internal region of FIV Matrix Protein p15
- 亚型
- IgG
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- 应用备注
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ELISA: 1/5,000 - 1/20,000. Western Blot: 1/1,000 - 1/10,000. Immunocytochemistry: 1/20 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.22 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- FIV P24 Gag
- 物质类
- Viral Protein
- 背景
- Feline immunodeficiency virus (FIV) belongs to the lentivirus family. This family is characterized by assembly of the viral capsid at either the plasma membrane or at the limiting membrane of late endosomes. The capsid assembles from the viral Gag polyprotein. Upon release of a budding virion, Gag precursor protein is cleaved by the viral protease into its mature products, namely Matrix Protein, Capsid and Nucleocapsid. Matrix Protein, located at the N-terminus of the Gag polyprotein, is usually myristylated during protein translation, prior to the later events of virus assembly. The myristate moiety is believed to be sequestered within the Matrix Protein during protein translation and later facilitates membrane binding upon exposure resulting from conformational changes. Essential functions attributed to the Matrix Protein of lentiviruses include targeting newly synthesized Gag precursor proteins to the site of virus assembly by binding with cellular components such as phosphatidylinositides. In the mature virus particle, the Matrix Protein provides internal structure to the virion within the capsid, but is not exposed at the surface of the particle. Based on studies with HIV, it is postulated that FIV Matrix Protein may also serve additional functions, including nuclear localization of the viral core upon entry of the virus into a new host cell.Synonyms: Feline Immunodeficiency Virus Matrix Protein p15, Gag polyprotein
- 基因ID
- 11674
- UniProt
- P16087
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