MLF1IP 抗体 (Thr78)
MLF1IP
适用: 人, Cow, 犬, Chimpanzee
WB, IHC (p), EIA, IF
宿主: 兔
Polyclonal
unconjugated
3 images
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- 抗原 See all MLF1IP 抗体
- MLF1IP (MLF1 Interacting Protein (MLF1IP))
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抗原表位
- Thr78
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适用
- 人, Cow, 犬, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MLF1IP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunofluorescence (IF)
- 特异性
- This antibody is specific for MLF1IP protein phosphorylated at Thr78.
- 交叉反应 （详细）
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Species reactivity (expected):Canine, Bovine, Chimpanzee, Rat, Mouse.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to amino acids surrounding Thr78 of human MLF1IP protein. The immunogen peptide is phosphorylated at Thr78
- 亚型
- IgG
- Top Product
- Discover our top product MLF1IP Primary Antibody
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anti-MLF1 Interacting Protein (MLF1IP) (N-Term) antibody
MLF1IP 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Immunocytochemistry: 1/100 - 1/500. Immunohistochemistry: 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.26 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- MLF1IP (MLF1 Interacting Protein (MLF1IP))
- 别名
- MLF1IP (MLF1IP 产品)
- 别名
- MGC147270 antibody, CENP50 antibody, CENPU antibody, CENPU50 antibody, KLIP1 antibody, PBIP1 antibody, CENP-U antibody, CENP-50 antibody, 1700029A22Rik antibody, centromere protein U antibody, MLF1 interacting protein antibody, cenpu antibody, MLF1IP antibody, CENPU antibody, Cenpu antibody
- 背景
- Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel pololike kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/ anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.Synonyms: CENP-U(50), CENPU, Centromere protein U, ICEN24, Interphase centromere complex protein 24, KLIP1, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, PBIP, Polo-box-interacting protein 1
- 基因ID
- 79682
- NCBI登录号
- NP_078905
- UniProt
- Q71F23
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