H2AFV 抗体 (pSer137)
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- 抗原 See all H2AFV 抗体
- H2AFV (H2A Histone Family, Member V (H2AFV))
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抗原表位
- AA 132-141, pSer137
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适用
- Drosophila melanogaster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This H2AFV antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against the phosphorylated form of Drosophila H2AvD protein at the pSer137 residue. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA. A BLAST analysis was used to suggest little to no cross reactivity with H2AvD proteins from other sources based on a comparison using the immunizing sequence. Expect a band approximately 14 kDa in size corresponding to phosphorylated H2AvD protein by western blotting in the appropriate Drosophila tissue or cell lysate or extract. Less than 0.2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pS137 of H2AvD protein.
- 纯化方法
- Affinity Chromatography: The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
- 免疫原
- Synthetic peptide corresponding to amino acids 132-141 of Drosophila melanogaster (fruit fly) H2AvD protein.
- 亚型
- IgG
- Top Product
- Discover our top product H2AFV Primary Antibody
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anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibody
H2AFV 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-128) antibodyH2AFV 适用: 人 ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (C-Term) antibodyH2AFV 适用: Drosophila melanogaster WB, ChIP 宿主: 小鼠 Monoclonal 10E9-D1 unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 3-128) antibodyH2AFV 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (N-Term) antibodyH2AFV 适用: 人, Drosophila melanogaster, Cow, 犬, 豚鼠, 马, 小鼠, 兔, 大鼠, 绵羊, 斑马鱼, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-H2A Histone Family, Member V (H2AFV) (AA 1-129) antibodyH2AFV 适用: 人 ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-H2A Histone Family, Member V (H2AFV) antibodyH2AFV 适用: Drosophila melanogaster ELISA, WB, ChIP 宿主: 小鼠 Monoclonal unconjugated Recombinant Antibody
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- 应用备注
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ELISA: 1/4,000-1/16,000. Western Blot: 1/400-1/1,600.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody (in aliquots) at-20 °C. This product is stable for several weeks at 2-0 °C as an undiluted liquid. Dilute only prior to immediate use.
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- 抗原
- H2AFV (H2A Histone Family, Member V (H2AFV))
- 别名
- Histone H2A.v (H2AFV 产品)
- 别名
- H2A.Z-2 antibody, H2afv antibody, H2AV antibody, h2av antibody, C530002L11Rik antibody, H2a.z-2 antibody, H2av antibody, H2A.F/Z antibody, h2afv antibody, h2afz antibody, H2A histone family, member V antibody, H2A histone family member V antibody, H2A histone family member V S homeolog antibody, H2A histone family, member Va antibody, H2afv antibody, LOC100226437 antibody, H2AFV antibody, h2afv.S antibody, h2afva antibody
- 背景
- Variant histones H2A are synthesized throughout the cell cycle and are very different from classical Sphase regulated H2A. H2AvD is vital for viability, but the exact function of variant histones H2A is not known. H2A is a core component of the nucleosome, an octamer containing two molecules each of H2A, H2B, H3 and H4. The octamer wraps approximately 146 bp of DNA. HsAvD is expressed both maternally and zygotically and is found in embryos through to adults (female only). The human homologue, H2AX, is phosphorylated by ATM protein kinase when double strand DNA breaks occur. In mouse, H2AX "knock out" mice have an increased incidence of cancer.Synonyms: CG5499, H2A.F/Z, H2AFV, H2AV, H2AvD, His2Av, His2AvD
- 基因ID
- 43229
- NCBI登录号
- NP_524519
- UniProt
- P08985
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