ELISA: 1: 4,000 - 1: 16,000. Western blot: 1: 500 - 1: 3,000. Immunohistochemistry on paraffin sections: 2 μg/mL to 20 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.46 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factorinduced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.Synonyms: AT225, EGR-1, ETR103, Early growth response protein 1, KROX-24, NGFI-A, Nerve growth factor-induced protein A, Protein Krox-24, Transcription factor ETR103, Transcription factor Zif268, Zif-268, Zif268